In this step we will visualize the gene regulatory network in Cytoscape 3. To load the network we must first ensure that NDB reader is installed in Cytoscape 3. First, click the Cytoscape 3 icon in the GenomeSpace toolbar to launch the program. Start a new session by selecting “With Empty Network”, and feel free to change the name, or simply leave as-is. Next, navigate to File>Import>Network>GenomeSpace. In this new window, select the .ndb file you would like to import. In this case, “stemness_network.ndb”. Once the file has loaded, Cytoscape will prompt you to import the network to a certain collection or location. Once you have made your selection, click “OK” in the next window to finalize your network view.