Next we will convert two files from the MACS output to the BigWig format for visualization in IGV. The two bedgraph files contain individual fragment pile up for the wild type and knock out samples. Select the pencil icon for the treatment peak enrichment output. Under the “Attributes” tab, assign the mouse reference genome mm9 from NCBI. Then, select “Save”. Select the “Convert Format” tab and “Convert bedgraph to BigWig”. Select “Convert”. Note: these steps will also have to be performed on the control peak enrichment file.